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ylbQ [2019-08-01 09:51:23]
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ylbQ [2019-08-01 09:51:23]

2-dehydropantoate 2-reductase
locus
BSU_15110
pI
5.86
mw
33.13 kDa
protein length
298 aa Sequence Blast
gene length
897 bp Sequence Blast
function
biosynthesis of coenzyme A
product
2-dehydropantoate 2-reductase
essential
no
ec
1.1.1.169
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.4|Biosynthesis of coenzyme A]

    • Gene

    Coordinates
    1,577,409 1,578,305

    The protein

    Catalyzed reaction/ biological activity

  • (R)-pantoate + NADP+ = 2-dehydropantoate + NADPH (according to Swiss-Prot)

    • Protein family

  • ketopantoate reductase family (with [protein|60264E4BE76F5995A8317294D05FC27F8B34E09D|YkpB], according to UniProt)

    • Structure

  • [PDB|3LGO]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|0F153803781C9AC8321FB14F926B645C45E53B00|ylbQ]-[gene|115FA333FAD17ACE5EBF288F16F33C330C6B4473|bshC]
    description
    [Pubmed|20308541]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|23894131], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [Pubmed|23894131], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]

    • regulation

  • ''[protein|search|ylbQ]'': induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]
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    Biological materials

    Mutant

  • MGNA-B254 (ylbQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1253 NBRP B. subtilis, Japan]
  • BKE15110 ([gene|0F153803781C9AC8321FB14F926B645C45E53B00|ylbQ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATGTCACACCCAATTT, downstream forward: _UP4_TGAGCTTTTTCGGTAACATG
  • BKK15110 ([gene|0F153803781C9AC8321FB14F926B645C45E53B00|ylbQ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATGTCACACCCAATTT, downstream forward: _UP4_TGAGCTTTTTCGGTAACATG

    • References

    Operon and expression

  • 20308541,23894131